Essential Residues
Residues such as Lys8, Phe23, Lys91 and Arg149 are involved in RNA binding.
Post-translational Modification
Serine 57 and 60 are phosphorylated by the host.
Structure of Protein M2-1 from HMPV
3D Structure
| Method | Downloads |
|---|---|
| AlphaFold 3 | Click here |
Functional Information
The M2-1 protein is a zinc binding protein encoded by the Human Metapneumovirus (hMPV) that plays a vital role in RNA synthesis regulation. M2-1 is a basic protein with 187 amino acids and a theoretical pl of 9.14. It is expressed only during the viral cycle's replication. M2-1 recruits the antitermination factor to the polymerase during transcription to bind nascent viral mRNA. Zinc binding activity is essential for viral replication and pathogenesis in vivo. (PubMed:15890897)
Interactions
The homotetramer interacts with RNA. Interacts with the phosphoprotein (P); this interaction is required for protein M2- 1 function, localization in host inclusion bodies. Interacts with the nucleoprotein (N). Interacts with the matrix protein (M); this interaction directs M localization to cytoplasmic inclusions comprising viral proteins L, N, P, and M2-1 and mediates M association with the nucleocapsid.
Domains Orgainzation
- Zinc Finger = 1-28.
- Oligomerization domain=32-49.
- Central globular core = 75-167.
Mutation
- C7S = 58% loss of zinc binding and loss of homotetramerization. Delayed viral replication.
- C15S = 58% loss of zinc binding and loss of homotetramerization. Delayed viral replication.
- C21S = Complete loss of zinc binding and loss of homotetramerization. Delayed viral replication.
- C25S = Complete loss of zinc binding and loss of homotetramerization. Delayed viral replication.